Antigen capsid-incorporation vector elicits an <i>in vivo T. cruzi</i> humoral immune response.

<p>BALB/c and C57BL/6 mice (n = 7) were primed, boosted, and reboosted with 1×10¹⁰ VP of Ad vectors. A) Immunization timeline showing when immunizations were performed (solid arrows) and sera was collected (dashed arrows). B) Post-prime, post-boost, and post-reboost BALB/c serum or C) Post-prime, post-boost, and post-reboost C57BL/6 serum was collected for ELISA binding assays. Ten µM of purified gp83 (KIYWKQPVEGTKSWTLSK) antigenic peptide was bound to ELISA plates. The plates were then incubated with serial diluted concentrations of immunized mice serum and the binding antibodies were detected with HRP conjugated secondary antibody. The amount of anti-gp83 antibodies in the sera was calculated based on a standard curve of mouse IgG protein. The values are expressed as the mean ± standard deviation. (*) = <i>P</i>≤0.05, and (**) = <i>P</i>≤0.01.