Profiles of serological markers of WHV infection and WHV DNA detection in serum, PBMC and liver tissue samples, and the results on liver histology in 4/F and 6/M woodchucks with POI and in a control 13F animal with SOI after challenge with a single 10¹⁰ virion dose of WHV/tm3.

<p>At 66 months post-injection with 100 or 10¹⁰ virions of WHV/tm3 (see <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004332#ppat-1004332-g001" target="_blank">Figure 1</a>), each woodchuck was challenged with 10¹⁰ WHV/tm3 virions. The appearance and duration of serum WHsAg and anti-WHc positivity, the estimated WHV DNA loads in sera, PBMC and liver autopsy samples, and liver morphological alterations were presented as described in the legend to <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004332#ppat-1004332-g001" target="_blank">Figure 1</a>.