Time-dependent phenotypic changes observed after application of honey to log phase <i>E. coli</i> cultures.

<p>The changes paralleled the growth kinetics shown in Fig. 1D. (A) lag phase <i>E. coli</i> culture, (B) log phase <i>E. coli</i> culture at the time of honey application, (C) <i>E. coli</i> phenotypes after 30 min incubation with honey H208 (12.5% v/v), (D). <i>E. coli</i> phenotypes observed after 1 h incubation with honey H210 (12.5% v/v), (F) and (G) <i>E. coli</i> spheroplasts formed after 2 h incubation with honey H208 and H210 at 50% v/v, (H) spheroplasts observed after 18 h incubation, lag phase. The size-scale is the same for all micrographs in the figure, obtained under the same 400x magnification.