SEM of <i>E.coli</i> spheroplasts generated by ampicillin and honey-treatments.

<p>Log-phase <i>E. coli</i> cells were treated with bactericidal concentrations of ampicillin (2.5 µg/ml) and honey H208 (25% w/v) for 3 hr at 37°C. Cells were harvested and glutaraldehyde-fixed (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0106967#s4" target="_blank">Materials and Methods</a>). (A). <i>E. coli</i> control, (B). <i>E. coli</i> cels after treatment with ampicillin and (C). <i>E. coli</i> cell after treatment with honey and (D) honey-induced spheroplast lysis.