Effect of honey bee larval bodily fluid on <i>Paenibacillus larvae</i> growth.

<p>Effect of different concentrations of honey bee larval bodily fluid on the <i>in vitro</i> growth of <i>P. larvae</i> bacterial cells, expressed as the optical density measured at a wavelength of 590 nm (OD₅₉₀) in function of time (hours). Growth alterations were determined for six bodily fluid concentrations, expressed as the fold dilution in BHIT broth cultures: 10× dilution (♦), 25× dilution (▪), 50× dilution (▴), 100× dilution (⋄), 250× dilution (□), 500× dilution (▵), control (○). Each point in the graph displays the mean of three independent replicates, with the error bars being the standard deviations. Time 0 represents the time of spiking. Trend lines are calculated with DMfit. No OD measurements were performed during lag phase.