A398 induces apoptosis in HL-60 cell line.

<p>Cells were treated with A398 (4, 6 and 8 µM) or etoposide (5 µM) for 1 h, 3 h or 6 h and stained with annexin V-alexa fluor 488 and PI or with TMRM. The rate of apoptosis (A) and the percentage of depolarized cells (B) were quantified by flow cytometry. (C) Decreased expression of Bcl-2 in HL- 60 lineage. Cells were treated with 6 µM of A398 for 1 h, 3 h, 6 h and 12 h. The bands were revealed on nitrocellulose membrane after staining with secondary antibody labeled with peroxidase. β-actin was used as a loading control. C: control. (D) Bar plot showing arbitrary unit (AU) obtained from densitometry measure displayed in (C). Data are expressed as mean ± SE of three independent experiments. *p<0.05, **p<0.01 and ***p<0.001 compared with the control by ANOVA.