NUC-7738 induces apoptosis in AML cell lines.

OCI-AML3 and U937 cells were treated with NUC-7738 at 5, 10 and 20 μM for 48 hrs and apoptosis was determined by Annexin-V DAPI staining. (A) dot plots generated during flow cytometry analysis highlighting the percentage of live (LL), early apoptotic (LR), late apoptotic (UR) and dead (UL) cells. (B) Bar graphs highlight the proportion of cells which are viable, in early apoptosis or in late apoptosis as determined by Annexin-V / DAPI staining, and analysis by flow cytometry. The percentages shown indicate the proportion of total events within each of the respective categories as gated on the flow cytometry plots. Gating strategy highlighted in S1 Fig in S1 File. Each bar represents the mean percentage from three independent experiments with error bars indicating SD showing NUC-7738 caused a reduction in viable cells and an increase in early and late apoptotic cells in all cells examined. NUC-7738 did not induce apoptosis in the KG1a cell line (S20 Fig in S1 File).