T-plastin favors keratinocyte migration through FAK and β6-integrin.

<p>(<b>a</b>) FAK protein expression was quantified by western blot analysis in NCTC (3 independent experiments) or primary keratinocytes (n = 3) after FK506 exposure for 24 h and after siRNA transfection (<b>b</b>) β6-integrin mRNA expression was quantified by quantitative RT-PCR in NCTC (3 independent experiments) or primary keratinocytes (n = 3) after FK506 treatment for 4 or 24 h and results are expressed as ratio relative to untreated control taken as 1. (<b>c</b>) β6-integrin mRNA expression was evaluated after siRNA transfection by qRT-PCR (3 independent experiments). All results are means ± SD. The values reported under each blot are the mean fold protein expression relative to controls taken as 1 after normalization by GAPDH (Image J quantification). *<i>p</i><0.05, **<i>p</i><0.01.