Loss of ColXVII leads to EGF-induced S1356 phosphorylation of the β4 integrin subunit.

<p><b>A</b>, Normal human keratinocytes (NHK) and ColXVII-deficient JEB keratinocytes were lysed and equal amounts of total protein were immunoblotted with the indicated antibodies. The graph combines the quantification of NHKs (four donors) and JEB keratinocytes (four patients). <i>*p</i><0.05. <b>B</b>, This graph shows the quantification of β4 integrin subunit S1356 phosphorylation in NHKs and ColXVII-deficient JEB keratinocytes relative to total β4 integrin subunit expression levels. <i>*p</i><0.05. <b>C</b>, Sub-confluent <i>Col17a1⁻</i>^{<b><i>/</i></b><i>−</i>} keratinocytes with β4 integrin knockdown (β4 kd#1 and β4 kd#2) were treated with recombinant EGF [200 ng/ml] for 10 minutes, lysed and immunoblotted with indicated antibodies. Quantification is shown as mean ± SEM (number of independent measurements = 3, including two clones with β4 knockdown and a mock control); <i>*p</i><0.05.