Western blotting analysis of E7E6, p53 and pRb expression.

<p>The lysates of 293 cells transfected with pCTLA4-E7E6, pE7E6, pwCTLA4-E7E6, pwE7E6, pwE7, pwE6, pVAX1 or phosphate buffered saline (PBS, labeled as None) were probed with mouse anti-E7, goat anti-E6, mouse anti-p53, rabbit anti-pRb, or mouse anti-β-actin antibody. β-actin served as loading control. pCTLA4-E7E6 encodes CTLA-4 and mutant E7E6 fusion protein. pE7E6 encodes mutant E7E6 fusion protein. Accordingly, pwCTLA4-E7E6 and pwE7E6 carry wild type E7E6 fusion gene. pwE7 and pwE6 encode wild type E7 and E6 respectively. Expressed recombinant proteins were labeled by oblique arrows. MW: molecular weight. The lowest panel showed the relative expression levels of p53 and pRb by normalizing the signal intensities to actin.