Downregulation of p53 in transformed fibroblasts expressing E6 HPV transcripts.

<p>(A) E6 HPV18 and E6 HPV16 RNA quantification from HPFs, parental cells and fibroblasts transformed by apoptotic HeLa cells or apoptotic Ca Ski cells (named FH and FC respectively) was performed by real-time quantitative PCR following reverse transcription. The graphs represent the mean of three independent experiments (+/− SD). (B) Immunoblotting analyses of p53 and p21 expression in cancer cell lines, HPFs and FH and FC transformed fibroblasts, using mouse monoclonal antibodies against p53 and p21. Blots were also probed with a β-actin antibody.