Adseverin knockdown osteoclasts fail to resorb mineralized matrix.

<p><b>A</b>) Luc KD and Ads KD osteoclast precursors were cultured on dentin discs for 4 days. Mature Luc KD osteoclasts were capable of resorbing the dentin disc substrate (black arrow) significantly better than Ads KD cells, as indicated by the total resorbed area. Ads KD Clone F cultures had virtually no multinucleated osteoclasts or detectable resorption pits. <b>B</b>) WT and Ads KD (Clone F) cells were cultured for 2 days with sRANKL. More than 80% of WT cells were TRAcP. WT mononuclear TRAcP positive osteoclasts were capable of minimally resorbing the dentin slices as indicated by the small resorption pits (black arrows). Immature, Day 2 Ads KD cultures were TRAcP negative and displayed no resorptive ability.