Mononuclear precursors of human osteoclasts are found in the CD14(+) monocyte fraction of circulating peripheral blood mononuclear cells (PBMCs). It is possible to generate osteoclasts in vitro from PBMCs cultured with macrophage colony-stimulating factor and receptor activator for nuclear factor κB ligand. In these cultures, however, it is not possible to distinguish the effect of a specific agent on osteoclast resorption activity as opposed to osteoclast differentiation. To produce a population of mature human osteoclasts to study osteoclast lacunar resorption specifically, we cultured CD14(+) human monocytes on hydrophobic dishes in order to generate and maintain osteoclasts in suspension prior to culturing them on coverslips and dentine...
Mononuclear osteoclast precursors are present in the wear-particle-associated macrophage infiltrate ...
Monocytes and macrophages are capable of degrading both the mineral and organic components of bone a...
The bone resorbing osteoclasts are a complex type of cell essential for in vivo bone remodeling. The...
Mononuclear precursors of human osteoclasts are found in the CD14(+) monocyte fraction of circulatin...
The osteoclast is known to be formed by fusion of circulating mononuclear precursor cells of haemato...
Osteoclasts (OCs), the bone-resorbing cells, play a key role in skeletal development and adult bone ...
Osteoclasts are cells of hematopoietic origin with a unique property of dissolving bone; their inhib...
Objectives: Peripheral blood mononuclear cells (PBMC) are an attractive source for the generation of...
Osteoclasts are multinucleated cells that carry out bone resorption. Analysis of the direct effect o...
Research involving osteoclasts has always been difficult to undertake owing to a lack of osteoclast ...
Item does not contain fulltextOsteoclasts are multinucleated cells derived from the hematopoietic mo...
Bone remodeling can be mimicked in vitro by co-culture models. Based on bone cells, such co-cultures...
Bone remodeling can be mimicked in vitro by co-culture models. Based on bone cells, such co-cultures...
Bone remodeling can be mimicked in vitro by co-culture models. Based on bone cells, such co-cultures...
<p>Isolated PBMC were differentiated in the presence of Macrophage Colony-Stimulating Factor (M-CSF)...
Mononuclear osteoclast precursors are present in the wear-particle-associated macrophage infiltrate ...
Monocytes and macrophages are capable of degrading both the mineral and organic components of bone a...
The bone resorbing osteoclasts are a complex type of cell essential for in vivo bone remodeling. The...
Mononuclear precursors of human osteoclasts are found in the CD14(+) monocyte fraction of circulatin...
The osteoclast is known to be formed by fusion of circulating mononuclear precursor cells of haemato...
Osteoclasts (OCs), the bone-resorbing cells, play a key role in skeletal development and adult bone ...
Osteoclasts are cells of hematopoietic origin with a unique property of dissolving bone; their inhib...
Objectives: Peripheral blood mononuclear cells (PBMC) are an attractive source for the generation of...
Osteoclasts are multinucleated cells that carry out bone resorption. Analysis of the direct effect o...
Research involving osteoclasts has always been difficult to undertake owing to a lack of osteoclast ...
Item does not contain fulltextOsteoclasts are multinucleated cells derived from the hematopoietic mo...
Bone remodeling can be mimicked in vitro by co-culture models. Based on bone cells, such co-cultures...
Bone remodeling can be mimicked in vitro by co-culture models. Based on bone cells, such co-cultures...
Bone remodeling can be mimicked in vitro by co-culture models. Based on bone cells, such co-cultures...
<p>Isolated PBMC were differentiated in the presence of Macrophage Colony-Stimulating Factor (M-CSF)...
Mononuclear osteoclast precursors are present in the wear-particle-associated macrophage infiltrate ...
Monocytes and macrophages are capable of degrading both the mineral and organic components of bone a...
The bone resorbing osteoclasts are a complex type of cell essential for in vivo bone remodeling. The...