Knockdown of JMY during meiotic maturation.

<p><b>A–D</b>: GV oocytes injected with dsRNAs or control were cultured for 44 hours. Knockdown of JMY mRNA was determined by RT-PCR (<b>A</b>) and Western blotting (<b>B</b>). Subcellular localization (<b>C</b>) and quantitized fluorescence intensity (<b>D</b>) of JMY fluorescence of dsRNA or control injected oocytes measured at MI (20 h after culture) and MII (44 h after culture) stages respectively. <b>E</b>: Germinal vesicle breakdown (GVBD) and polar body extrusion (PBE) rates of JMY dsRNA induced oocytes. Red: JMY, Blue: chromatin. Values represent mean ± s.e.m. *<i>p</i><0.05, n = 5.