Effects of meiotic checkpoint activation on DmRad9A oocyte nuclear membrane localization.

<p>Confocal images of stage 7 egg chambers. (A, E, I and M) are stained for DNA (blue, arrows mark oocyte nucleus DNA, karyosome); arrows mark the oocyte nucleus (karyosome). (B, F, J and N) GFP-DmRad9A (green). (C, G, K and O) are stained with anti-lamin antibodies, which mark the nuclear membrane, in red. (Inset in H, L and P), represents a schematic description of the oocyte nucleus. Red-lamin, green-GFP-DmRad9A and blue-karyosome. (A–H) GFP-DmRad9A:: <i>nosGal 4-VP16</i> egg chamber, E–H are enlargement of the oocyte region from A–D, respectively. (I-L) BAF3A:: GFP-DmRad9A:: <i>nosGal 4-VP16</i> egg chamber. M–P, GFP-DmRad9A:: <i>nosGal 4-VP16; okr^AA</i>/<i>okr^RU</i>.