Knockdown of <i>RPL31</i> inhibits cell-cycle progression.

<p>(A) Knockdown of <i>RPL31</i> in BicR cells increased the proportion of cells in G0/G1 and decreased the proportion of those in S phase. Cells were transfected with siRPL31 or siLuc in culture medium for 48 h. Cells were then washed with PBS, stained with propidium iodide, and subjected to FACS analysis. (B) The percentages of BicR cells in S, G0/G1, and G2/M phase were determined using CellQuest software and are shown as mean ± s.d. (n = 3; *, <i>P</i><0.05; **, <i>P</i><0.01). (c) Knockdown of <i>RPL31</i> decreased the proliferation of LNCaP cells. Cells were treated the same as described in (A). (D) The percentages of LNCaP cells in S, G0/G1, and G2/M phases were determined using CellQuest software and are shown as mean ± s.d. (n = 3; **, <i>P</i><0.01).