Cellular effects of differentially expressed RPS27L in LoVo cells.

<p>(A) Cell cycle regulation. Cell phases were determined following an indicated VP16 treatment. (B) Cells with newly synthesized DNA under VP16 treatment. The percentage of BrdU-positive cells (blue spots) was determined following an indicated VP16 treatment. (C) Annexin-V/PI double-staining assay. Arrows indicated annexin V-FITC and PI positive cells. VL, visible light; PI, propidium iodide. Green spot, annexin V-FITC; red spot, PI. (D) Cells with JC-1 stain. Arrows indicated the red granular pattern of JC-1 aggregation. (E) Immuno-detection of PARP cleavage. Cleaved PARP, 89 kDa; GAPDH, 36 kDa. shLuc, RPS27L-expressing cells; shRPS27L, RPS27L-lacking cells; overRPS27L, RPS27L-overexpressing cells.