RPL31 regulates levels of p53 protein expression.

<p>(A) Knockdown of RPL31 increases p53, MDM2, and p21 protein expression. LNCaP and BicR cells were transfected with siRPL31 or siLuc for 48 h. Cell extracts were subjected to SDS-PAGE and western blot analysis using the indicated antibodies. (B) RPL31 regulated the degradation of p53 protein. BicR cells were transfected with siRPL31 or siLuc for 60 h and treated with 50 µg/mL cycloheximide (CHX) for the indicated time. Cell extracts were analyzed by western blotting. (C) p53 protein levels were quantified by densitometry and normalized to the levels of the corresponding β-actin protein and shown as mean ± s.d. (n = 3; *, <i>P</i><0.05).