Untargeted metabolomics demonstrate production of odd-carbon-length fatty acids in mice fed triheptanoin diet.

<p><b>A.</b> Heat map representation of relative levels of odd-carbon-length fatty acids in liver. <b>B.</b> Heat map representation of relative levels of odd-carbon-length fatty acids in skeletal muscle. SBO, soybean oil control diet. Metabolomics data were normalized to the median of the WT+chow control group, median scaled to 1, but are presented as averages in the heat maps. Row-normalized heat maps display individual samples by column and metabolites by row. Color intensity is scaled within each row so that the highest expression value corresponds to bright red and the lowest to bright green. All fatty acids in Figure 9 showed significant effects of diet by two-way ANOVA of contrasts (p <0.0001).