Behavior of C17.2 cells cultured in 2000 µm microchannels with 24, 48 and 96 hour feeding intervals.

<p>(A) The β-tubulin-III positive cell counts per mm² over time. (B) The neuronal cell counts per mm² over time. (C) The average neurite length per mm² over time. Groups lacking neuronal cells were labeled with “O” in Figure 5B and Figure 5C. The control (with “c”) was C17.2 NSCs seeded at the same surface density in FluoroDishes but without microchannels and fed every 48 hours as in standard subculture protocols. The * above the bars indicated a statistical difference between the sample and the control by two-tailed Student’s t test (<i>p</i><0.05). The * below the bars indicated a statistical difference in the group by one way ANOVA (<i>p</i><0.05). The ** in Figure 5C indicates a significant difference due exclusively to the samples with no neurite outgrowth. N≥15.