Ded1 destabilizes docking of the P1 helix.

<p>(A) Representative FRET traces showing extended undocked lifetimes before redocking (left) and unwinding (right) in the presence of Ded1 and ATP (transitions shown in red). (B) Lifetime plots of the undocked states in the absence of Ded1 (black, all panels), with 50 nM (blue) or 0.2 µM (green) Ded1 and 2 mM ATP (left panel), with 0.1 µM (cyan) or 0.9 µM (orange) Ded1 and 2 mM AMP–PNP (center panel), and with 0.9 µM Ded1 and no nucleotide (red, right panel). (C) Lifetime plots of the docked state of P1 under the same conditions and represented by the same color scheme as (B). See also <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001981#pbio.1001981.s013" target="_blank">Data S1</a>. The calculated <i>k</i>_dock and <i>k</i>_undock values for each condition are listed in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001981#pbio.1001981.s012" target="_blank">Table S5</a>.