Representative time courses of dissociation of the head from MT after T → D switch.

<p>(A) The probability of the KIF1A head still attached on MT as a function of duration time from the T → D switch. The red, blue, orange, and green lines correspond to the cases of [cargo/strong], [cargo/weak], [stand-alone/strong], and [stand-alone/weak], respectively. The inset is the corresponding results of [cargo/strong] with the three sizes of cargoes, a smaller cargo with friction 2.5×10⁻⁸ (light-green), a middle one with 1.1×10⁻⁸ (red), and a larger one with 0.53×10⁻⁸ (magenta). (B)(C) The binding energy between the KIF1A head and MT as a function of duration time from the T → D switch for case of the strong interaction (B), and for the case of the weak interaction (C). (D) A dissociation trajectory in the plane (E_B, z_relative) after the T → D switch, where E_B is the binding energy between KIF1A and MT, and z_relative is the position of the cargo-analog (the C-terminal) relative to that of the head (i.e., z_cargo-z_head). The color assignments in (B) (C) (D) are the same as those in (A).