Modelisation of the dynamics of individual MTs.

<p>(<b>A</b>)<b>.</b> Scheme of a dynamic MT (green) elongating from a stable MT seed (red). The different parameters of MT dynamics are (i) growth rates at (+/−) ends (V_G⁺/V_G⁻), (ii) shrinkage rates (V_s⁺/V_s⁻), (iii) catastrophe/rescue frequencies at MT (+) ends (f_res⁺/f_cat⁺) and (−) ends (f_cat^−/f_res⁻). (<b>B</b>)<b>.</b> Experimental kymograph of an individual MT fluctuating away from the seed (dark band at the kymograph center). Scheme on right of the kymograph shows the orientation of MT ends. (<b>C</b>)<b>.</b> History plots of two individual MTs observed by TIRFm. Blue and green traces correspond respectively to MT (+) and (−) ends. (<b>D</b>)<b>.</b> Kymograph of two simulated independent MTs elongating from a seed (red). MT (+) ends are in blue; MT (−) ends are in green. (<b>E</b>)<b>.</b> Corresponding fluctuation plots over time (same code color as in C).