ATP depletion leads to increased chromatin compaction.

<p>(A) FLIM images show the reduction of fluorescence lifetime in Hela^H2B-2FP cells upon ATP depletion and an increase when the drugs are washed away. The increase in compaction is also seen in the intensity images (Scale bar –10 µm). (B) The chart shows the average decrease and subsequent increase in EGFP fluorescence lifetime values normalised to the lifetime of the control nuclei. Error bars indicate standard deviation for values collected for 13 cells from 3 independent experiments. (* p-value = 3.7×10⁻⁸,+p-value = 0.0046).