Go6983 and GF109203x decreased Akt phosphorylation at both Ser⁴⁷³ and Thr³⁰⁸ in CHO-HCA₂ and A431 cells.

<p>Both CHO-HCA₂ cells (A and C) and A431 cells (B, D and E) were treated with either 10 µM Go6983 or 10 µM GF109203x for 1 h and Akt phosphorylation at Ser⁴⁷³ (A and B) and Thr³⁰⁸ (C–E) were detected. The data shown are representative of at least three independent experiments. The data were analyzed using Student’s t test (**, p<0.01; ***, p<0.001).