G-1 inhibits the phosphorylation of Akt and ERK1/2 in CASMCs.

<p>A and B: HCASMCs were cultured in serum and phenol red free medium for two days followed by PDGF-BB (10 ng/ml) (A) or G-1 (1 µM) treatment (B) for indicated time in the presence of 10% FCS. C: PCASMCs were cultured in the same medium for two days followed by G-1(10⁻⁷–10⁻⁵M); PDGF-BB (10 ng/ml); and G-1(10⁻⁷–10⁻⁵M) plus PDGF-BB (10 ng/ml) treatments for indicated time in the presence of 10% FCS. Total cell extracts (1×10⁶) were subjected to Western-blot analyses for Phospho-Akt and ERK1/2 level. Under the Western blot panels, a quantitative representation of the expression analysis from 3 independent experiments is shown. Vehicle-treated CASMCs cells were used as control. Data are normalized by β-Actin and expressed as means ±SD (n = 3). A significant difference is indicated by either **** p<0.0001, *** p<0.001, ** p<0.01 or * p<0.05(one or two-way ANOVA); C, G and P represent control, G-1 and PDGF-BB treatment sample respectively, ns indicates no significant difference.