HSA restores AKT phosphorylation at both threonine 308 and serine 473 residues in GML treated cells.

<p>APBTs were treated with 0.1% ethanol vehicle control in serum free media (solid black lines), 10 μg/ml GML in serum free media (solid grey lines), 0.1% ethanol vehicle control in 1% HSA (dotted black lines), or 10 μg/ml GML in 1% HSA (dotted grey lines). Cells were stimulated by crosslinking 2 μg/ml of anti-CD3 for various times. Phosphorylation of AKT at threonine 308 and serine 473 were assessed by immunoblotting. Representative blots <b>(A)</b> and immunoblot quantification <b>(B)</b> are shown from 5 independent experiments with different individual donors. # denotes p<0.05 in Student t’s test comparing ethanol and GML treated cells in serum free media. * denotes p<0.05 in Student t’s test comparing GML treated cells in serum free RPMI with GML treated cells in 1% HSA.