Robo2 and Abl alter cell-cell adhesion to control CySC maintenance.

<p>(A–F) Confocal sections of testes with Zfh-1 staining CySCs and early cyst cell daughters (red). Positively marked mosaic clones are identified by presence of GFP (green). At 8 days ACI (A) control <i>Abl⁴</i> CySCs (arrowheads) are present at high numbers in each testis, while (B) the number of <i>Abl⁴</i> CySCs expressing ECad RNAi (arrowhead) remains low. At 10 days ACI, (C) marked <i>Abl⁴</i> CySCs (arrowheads) are present in high numbers per testis, while (D) the number of <i>Abl⁴</i> CySCs expressing β-cat RNAi (arrowhead) remains low. At 2 days ACI, (E) <i>robo2</i> null GSCs (asterisk), but not CySCs are present while (F) <i>robo2</i> null CySC expressing Abl RNAi (arrowhead) are present in the niche and produce cyst cell daughter cells (arrows). Hubs outlined in white, DNA stained with DAPI (blue), scale bars = 10 µm.