<i>Psc</i> and <i>Su(z)2</i> are required in the CySC lineage for repression of <i>Abd-B.</i>

<p>(<b>A–B’’’</b>): Immunofluorescence images of testes from (A–A’’’) newly eclosed males with wild-type control or (B–B’’’) <i>Psc</i> and <i>Su(z)2</i> double mutant clones generated in the CySC lineage (<i>C587-GAL4;FRT42D, Df(2R)Su(z)2^1.b8/FRT42D, ubi-nGFP;UAS-FLP/tub-GAL80^ts)</i>, immunostained with anti-ABD-B (red), anti-Vasa (A', A’’, yellow), anti-GFP (B', B’’, green), and DAPI (blue). Arrow marks nucleus of a sheath cell. (<b>C–D’’’</b>): Testes with simultaneous RNAi knockdown of both <i>Psc</i> and <i>Su(z)2</i> in the CySC lineage (<i>C587-GAL4;UAS-Psc-RNAi/UAS-Su(z)2-RNAi;tub-GAL80^ts</i>) immunostained with anti-ABD-B (red), anti-Vasa (C', C’’, yellow), anti-Zfh-1 (D', D’’’ green), anti-Tj (D’’, grey), and DAPI (C’’’, blue). Scale bars: 50 μm.