TNF-α production by NK cells.

<p>(A) TNF-α production in peripheral blood [control subjects (n = 21), LCL patients (n = 28) and DCL patients (n = 6)]. (B) Analysis of TNF-α production of LCL patients according to gender [female (n = 11) and male (n = 17)]. (C) Double immunohistochemistry (CD57⁺/TNF-α⁺) staining of lesions of LCL and DCL patients showed dark green staining induced by the combination of a green substrate (Stay Green/AP) used for NK cells and DAB (brown) used for TNF-α staining. □ Non-stimulated NK cells, ▪ LPG-stimulated NK cells. Mean ±SEM is shown. *p≤0.05 was considered significant. Scale bar = 50 µm. Double positive cells CD57⁺/TNF-α⁺ (black arrows) and single positive cells CD57^-/TNF-α⁺ (red arrows).