Phenotype of NK cells stimulated with IL-2 or IL-15.

<p>PBMCs from two healthy normal donors (N1 and N2) and from the patient (P) (<b>A</b>, <b>B</b>) or sorted CD56^dim and CD56^bright NK cells from one healthy normal donor and from the patient (<b>C</b>) were stimulated for 3 days with IL-2. Also, PBMCs from two healthy normal donors (N1 and N2) and from the patient (P) were stimulated for 3 days with IL-15 (<b>D, E</b>). Thereafter, cells were harvested and stained with anti-CD3 and anti-CD56, and a third fluorochrome-labeled mAb (indicated on the <i>y</i>-axis). Also, expression of CD57 and CD158 in NK cells (gated as CD3⁻CD56⁺ cells) from N1, N2 and the patient (P), and on CD3⁻CD56^dim cells from P was plotted for cells stimulated with IL-2 or IL-15 (<b>B, E</b>). Expression of CD57 and CD158 on sorted and IL-2-stimulated CD56^dim NK cells from one healthy normal donor and from the patient is also shown (<b>C</b>). Data are depicted as dot plots. The percentages in each quadrant are shown. Results correspond to one from 3 independent experiments.