EV71 infection-induced oxygen consumption is associated with a reduction in respiratory efficiency.

<p>(A) SF268 cells were mock- or infected with EV71 at an m.o.i. of 1.25 for indicated times. Oxygen concentration was assayed with Clark oxygen electrode, and oxygen consumption rate (10⁻² µg O₂/5×10⁵ cells/min) was calculated accordingly. Results are mean ± SD, n = 6. *p<0.05 vs. uninfected cells. (B) SF268 cells were mock- or infected with EV71 at an m.o.i. of 1.25, and were treated without or with oligomycin. Oxygen concentration was assayed with Clark oxygen electrode, and oxygen consumption rate (10⁻² µg O₂/5×10⁵ cells/min) was calculated. Results are mean ± SD, n = 6. *p<0.05 vs. uninfected cells; ^#p<0.05, oligomycin-treated vs. untreated cells. (C) Oligomycin-sensitive oxygen consumption rate was calculated as the difference in the absence and presence of oligomycin. (D & E) The oxygen consumption was measured as described in (A) and (B). Data were normalized to the relative mitochondrial mass unit (MMU) of control and infected cells. Results are mean ± SD, n = 6. *p<0.05 vs. uninfected cells. (F) Oligomycin-sensitive oxygen consumption rate was calculated as described in (C), and normalized to the relative mitochondrial mass unit (MMU) of control and infected cells. (G–I) SF268 cells were mock- (−) or infected (+) with EV71 at an m.o.i. of 1.25 for 48 hr, and mitochondria were isolated and assayed for oxygen consumption rates (10⁻¹ µg O₂/mg mitochondrial protein/min) during state 3 (G) and 4_o (H) respiration as described in <i>Materials and Methods</i>. RCRs were calculated accordingly, and are shown (I). Results are mean ± SD, n = 6. *p<0.05 vs. uninfected cells.