Mitochondrial ROS are essential to EV71 replication.

<p>(A) SF268 cells were mock- (−) or infected (+) with EV71 at an m.o.i. of 1.25, and treated without (<i>Con</i>) or with indicated concentrations of Mito-TEMPO. Forty-eight hours later, cells were subject to MitoSOX Red staining and flow cytometric analysis. The mean fluorescence intensity (MFI) of MitoSOX of mock- and infected cells is expressed as the percentage of that of uninfected cells. The results are presented as mean ± SD, n = 3. *p<0.05 vs. infected Con group. (B) SF268 cells were mock- or infected with EV71 at an m.o.i. of 1.25, and treated without or with 200 µM of Mito-TEMPO. Forty-eight hours later, cells were harvested for western blotting with antibodies to phosphorylated eIF2α and total eIF2α, viral protein 3D, and actin. A representative experiment out of three is shown here. (C) SF268 cells were mock- (−) or infected (+) with EV71 at an m.o.i. of 1.25, and treated without (<i>Con</i>) or with 200 µM of Mito-TEMPO. Forty-eight hours later, cells were analyzed for levels of EV71 genomic RNA. The results are presented as means ± SD n = 3. *p<0.05 vs. infected Con group.