Activities of multimerised ELK3 target sites.

<p>(A) Schematic representations of the region of pGL3-Control that contains luciferase coding sequences, the initiation site of the SV40-early promoter and the site of insertion of 4 or 9 copies of S1, S2, S1 mut (S1m) or HIF1α target sequences (Bruning <i>et al</i>,) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0113050#pone.0113050-Bruning1" target="_blank">[20]</a>. (B) Effect of miR-155 mimic expression on luciferase expression from the indicated reporters, normalised to the control vector. The error bars represent the S.D. between two separate transfection experiments. (C) Block of miR-155 inhibition of endogenous ELK3 mRNA by overexpression of ELK3 S1 (site 1 miR-155 target sequence). miR-155 mimic or miR control were cotransfected with either the empty control vector or pGL3C-9xS1. Two independent transfection experiments, each analysed twice by qPCR, as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0113050#s2" target="_blank">Methods</a> section. **p<0.01, error bars represent S.D.).