MiR-29b suppresses the expression of the luciferase reporter with hPGRN 3′UTR.

<p>(<b>A</b>) Schematic representation of the constructs used in the luciferase reporter assay. hPGRN 3′UTR was cloned into the luciferase vector containing the SV40 promoter, which was cotransfected with the vector encoding pre-miR-29b-1 and H1 promoter. (<b>B</b>) Both pre-miR-29b-1 and pre-miR-29b-2, two genes located on different chromosomes that produce the identical mature miR-29b, had similar effects on luciferase expression. (<b>C</b>) Coexpression of miR-29b but not miR-9 suppressed the expression of luciferase with hPGRN 3′UTR. miR-29b also suppressed the expression of luciferase with mPGRN 3′UTR. (<b>D</b>) Luciferase activity was also reduced by a miR-29b mimic but not by a miR-9 mimic. Cel-miR-67 (Dharmacon), which doesn't exist in mammals, was used as the control. Values are mean ± SEM. **<i>P</i><0.01. ***<i>P</i><0.001.