PEDF Treatment Does Not Affect Cell Proliferation After Striatal Ischemic Injury.

<p>Mice received daily injections of 50 mg/kg BrdU for seven days starting at the day of MCAO. (A) Representative images of cortex, striatum, subventricular zone (SVZ), and dentate gyrus of CSF and PEDF group. The dotted line in the images of SVZ indicates the area used for counting the cells (distance of 50 µm from the ventricles). Scale bar = 200 µm. (B–C) Graphs represent the number of BrdU-positive cells/mm² in striatum, cortex, SVZ, and dentate gyrus. We did not find any significant differences between CSF and PEDF groups. (B) We found a significant difference between ipsilateral and contralateral striatum for both PEDF (repeated measures ANOVA, F(1,7) = 76.994, p<0.001) and CSF (F(1,7) = 96.351, p<0.001). However, for cortex, there is a significant difference between ipsilateral and contralateral cortex for PEDF (repeated measures ANOVA, F(1,7) = 14.415, p = 0.007), but not for CSF (F(1,7) = 3.016, p = .124). (C) We did not find any significant differences between ipsilateral and contralateral SVZ and dentate gyrus. Data is represented as means ± SD. n = 5 for CSF, n = 4 for PEDF.