Proliferation in the SVZ and RMS following 6-OHDA injections into the SNc.

<p>BrdU was administered 2 hours before animals were killed (see <i>Protocol 1</i>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031549#pone-0031549-g002" target="_blank">Fig. 2A</a>), and BrdU+ cells in the SVZ and RMS were quantified. (A–D) Estimated number of BrdU+ (A) and Ki67-ir (C) cell bodies in the SVZ. Estimated number of BrdU+ (B) and Ki67-ir (D) cell bodies in the RMS. (E–G) Photomicrographs of BrdU-LI in the SVZ of control mice (E), and mice 7 (F) or 42 days (G) after 6-OHDA was administered to deplete striatal DA. (H–J) Photomicrographs of Ki67-LI in the SVZ of control mice (H), and mice 7 (I) or 42 days (J) after striatal DA denervation. (K, L) Estimated number of NeuN-ir cell bodies in the GCL (K) and GL (L) of control mice, and mice 7 or 42 days after 6-OHDA administration. In plots (A–D) and (K), (L), white bars = control animals (n = 4), grey bars = mice 7 days after 6-OHDA injection (n = 4), and black bars = 42 days after 6-OHDA administration (n = 4). Control animals received injections of 0.9% NaCl into the SNc. CC, corpus callosum; LV, lateral ventricle; St, striatum; SVZ, subventricular zone; 7D, 7 days post 6-OHDA injection; 42D, 42 days post 6-OHDA injection. Scale bar in G = 50 µm, applies E–J. * corresponds to <i>P</i><0.05.