Neurogenesis is not affected by miR-124 NPs treatment.

<p>(A) Illustration of a coronal slice of the mouse brain representing the areas used to evaluate neurogenesis in the SVZ (red rectangle 1) and the peri-infarct area (red rectangle 2). (B) Representative confocal images of BrdU (green) and DCX (red) staining observed in the SVZ of sham-operated animal, a mouse subjected to PT and saline-treated and a mouse subjected to PT and miR-124 NPs-treated, respectively. Scale bar: 20 μm. Total number of (C, F) doublecortin (DCX)-positive cells, (D, G) BrdU-positive cells and (E, H) DCX/BrdU-double positive cells in the SVZ (C-E) and peri-infarct area (F-H) of mice after sham surgery or PT and indicated treatment conditions. All data are expressed as medians with the 1^st and 3^rd quartile values, with the following number of animals included in each experimental group: sham n = 11, PT saline n = 6, PT void NPs n = 6, PT scramble-miR NPs n = 8, PT miR-124 NPs n = 8. *p < 0.05 <i>versus</i> sham group and **p < 0.01 <i>versus</i> sham group. Abbreviations: BrdU, 5-bromo-2'-deoxyuridine; NPs, nanoparticles; PT, photothrombosis; SVZ, subventricular zone.