PI3K inhibition decreases IGF-1-induced neuronal differentiation of hippocampal NSCs.

<p>Cells were pretreated with LY294002 or DMSO for 40 min and then stimulated by IGF-1. (<b>A</b>) MAP-2 immunofluorescence analysis of neuronal differentiation of hippocampal NSCs. Nuclei were counterstained with Hoechst (blue). Scale bar, 25 µm. (<b>B</b>) Quantification of the MAP2-positive neurons in (<b>A</b>), Data are expressed as means ± SEM. **, <i>P</i><0.01 compared with control; #, <i>P</i><0.01 versus the IGF-1 group. LY, LY294002.