Suppression of IR-induced neuronal differentiation signaling by PI3K inhibitor in C17.2 cells.

<p>C17.2 cells treated with the inhibitors of MEK (PD98059), p38 (SB203580), PKA (H-89) and PI3K (LY294002) at 10 μM for 2 hr, and then exposed to IR at 6 Gy and incubated at 37°C for 72 hr. To assess rate of neurite bearing cells, each 200 cells in three randomly taken images were analyzed by Image J software. IR-induced neurite outgrowth is not affected by inhibitors of MEK, p38 and PKA but was blocked by PI3K inhibitor, LY294002 (A). Level of β-III tubulin and mGluR1, and activation of AKT, p53 and STAT3 were analyzed by Western blot (B). The results represent the mean ± SD from triplicate data. **p < 0.01 vs naive group, ††p < 0.01 vs radiation only group.