<p>Viruses were produced by transfection of HEK293T cells and virions were pelleted from the clarified supernatants. (<b>A</b>) gp160/gp120 (top panel) and p27 (bottom panel) were detected by Western blot using antibody 3.11H and 2F12, respectively. (<b>B</b>) Relative Env incorporation into virions. The ratios of gp120 to p27 were calculated by phosphorimaging analysis. Data are presented as the gp120:p27 ratio of mutant virions (Rn) relative to the gp120:p27 ratio of SIVmac239 wild-type (WT) virions (R239). (C) Mutants YG, E4b, E6b and E7b were quantified in triplicate. Shown is the average of independent experiments, error bars indicate standard deviation.</p
<p>Virus was concentrated, lysed and subjected to western blotting. Gp120, gp41 and p24 were detecte...
AbstractSIVmac239 and SIVmac1A11 are wild-type viruses encoding Env proteins with full-length or tru...
<p>(A) Schematic representation of JRFL WT and four different SP mutations evaluated in this study. ...
<p>Viruses were produced by transfection of HEK293T cells and virions were pelleted from the clarifi...
<p>(<b>A</b>) Schematic representation of additional mutations within the cytoplasmic domain of gp41...
Viruses were produced by transfection into 293T cells, virions were chemically inactivated with AT-2...
<p>HEK293T cells were transiently co-transfected with SIVmac239 Env mutants and a GFP expression pla...
<p>293T cells were transfected with the indicated molecular clones. At 24<a href="http://www.plospat...
<p>Viruses were produced by co-transfection of 293T cells with combinations of Env deficient backbon...
<p>Viruses were produced by transfection of HEK293T cells and virions were pelleted from the clarifi...
<p>(A) Schematic representation of REJO WT and eight different SP mutations evaluated in this study....
<p>Viruses were produced by transfection of HEK293T cells and virions were pelleted from the clarifi...
<p>(A) Shown is one representative of two independent infectivity assay experiments. Infectivity was...
<p>cDNA copy numbers (black bars), p24 levels (gray bars) and TCID<sub>50</sub> (open bars) derived ...
<p>293T cells were transfected in parallel with wild-type SIV<sub>SMM-PBj</sub> and SIV<sub>FIV CA-p...
<p>Virus was concentrated, lysed and subjected to western blotting. Gp120, gp41 and p24 were detecte...
AbstractSIVmac239 and SIVmac1A11 are wild-type viruses encoding Env proteins with full-length or tru...
<p>(A) Schematic representation of JRFL WT and four different SP mutations evaluated in this study. ...
<p>Viruses were produced by transfection of HEK293T cells and virions were pelleted from the clarifi...
<p>(<b>A</b>) Schematic representation of additional mutations within the cytoplasmic domain of gp41...
Viruses were produced by transfection into 293T cells, virions were chemically inactivated with AT-2...
<p>HEK293T cells were transiently co-transfected with SIVmac239 Env mutants and a GFP expression pla...
<p>293T cells were transfected with the indicated molecular clones. At 24<a href="http://www.plospat...
<p>Viruses were produced by co-transfection of 293T cells with combinations of Env deficient backbon...
<p>Viruses were produced by transfection of HEK293T cells and virions were pelleted from the clarifi...
<p>(A) Schematic representation of REJO WT and eight different SP mutations evaluated in this study....
<p>Viruses were produced by transfection of HEK293T cells and virions were pelleted from the clarifi...
<p>(A) Shown is one representative of two independent infectivity assay experiments. Infectivity was...
<p>cDNA copy numbers (black bars), p24 levels (gray bars) and TCID<sub>50</sub> (open bars) derived ...
<p>293T cells were transfected in parallel with wild-type SIV<sub>SMM-PBj</sub> and SIV<sub>FIV CA-p...
<p>Virus was concentrated, lysed and subjected to western blotting. Gp120, gp41 and p24 were detecte...
AbstractSIVmac239 and SIVmac1A11 are wild-type viruses encoding Env proteins with full-length or tru...
<p>(A) Schematic representation of JRFL WT and four different SP mutations evaluated in this study. ...