Effect of different mutations in Env on incorporation into virions.
- Carolina Beltran-Pavez (6103037)
- Carolina B. Ferreira (6103040)
- Alberto Merino-Mansilla (781674)
- Amanda Fabra-Garcia (6103043)
- Maria Casadella (6103046)
- Marc Noguera-Julian (440725)
- Roger Paredes (180297)
- Alex Olvera (3343655)
- Isabel Haro (2148178)
- Christian Brander (64220)
- Felipe Garcia (5660647)
- Jose M. Gatell (5657728)
- Eloisa Yuste (5664688)
- Victor Sanchez-Merino (5653459)
Publication date
December 2018
DOI Publisher
Public Library of Science (PLoS)Abstract
Viruses were produced by transfection into 293T cells, virions were chemically inactivated with AT-2 and pelleted from the clarified supernatants. (A) SU (gp120) and Gag (p55, p41 and p24) proteins were detected by Western blotting using 447-52D and anti-p24 monoclonal antibodies. (B) Ratios of SU to Gag (p55+p41+p24) were calculated by using phosphorimaging analysis. Virion producer cells were lysed as indicated in the Materials and Methods section. C) SU and β-actin proteins were detected by Western blotting using 447-42D and anti-β-actin monoclonal antibodies. D) Ratios of SU to β-actin were calculated by phosphorimagiing analysis. Data for LR1-C1 are presented relative to the ratio found for AC10 _29 virions.</p
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