The gene expression pattern changes in bones xenografted with osteoinductive PCa cells.

<p><b>A.</b> Flow chart outlining experimental (blue) and bioinformatics (grey) steps adopted to define a complete (OB-BMST) and a curated (“Core” OB-BMST) stroma response signature (orange). The first two experimental steps constitute the tissue compartment-specific transcriptional profiling (TCTP). <b>B.</b> Heatmap showing differentially expressed probe-sets between xenografted (C4-2B and VCaP) and control (Ep156T, intact and sham) bones. The expression level is color-coded: low expression is represented in blue, whereas high expression is represented in red. <b>C.</b> Venn diagram showing the number of overlapping and unique genes differentially expressed in C4-2B (FDR = 1E-05) and VCaP (FDR = 3E-05) xenografted bones. The sum of differentially expressed genes is referred to as the OB-BMST. <b>D.</b> Scatter plot showing log₂ fold change of differentially expressed genes in C4-2B and VCaP xenografts. <b>E.</b> Top 30 up-regulated genes of the OB-BMST derived from C4-2B (black bars) and VCaP (grey bars) xenografted bones.