The expression level of osteoblastogenic marker genes during ascorbic acid induced osteoblast differentiation.

<p>A) The expression of alkaline phosphatase (ALP), an early stage maker gene of osteoblast differentiation, as determined by real time RT-PCR. B) The expression of osteoclacin, a late stage maker gene of osteoblast differentiation, as determined by real time RT-PCR. Values (means ± SEM; n = 6) are presented as fold change from day 0 (untreated cells), data were analyzed using one-way ANOVA (GraphPad Prism6). A = <0.05 (from day 0), B = <0.05 (from day 4), C = <0.05 (from day 6), D = <0.05 (from day 8), E = <0.05 (from day 13), and F = <0.05 (from day 19).