Gating strategy used for flow cytometric analysis of H1-induced T cell cytokine expression.

<p>The plots show sequentially the gating hierarchy of one representative sample: CD3+ T cells, single cells, lymphocytes, time, CD8-T cells, which were further gated for CD4+ cells. The plots on the lowest row include the cytokine expression gates for either unstimulated or H1 fusion protein stimulated PBMC at day 70 divided in the co-expression of IL-17 and IL-2 or TNF-α and IFN-γ by CD4+ T cells.