Gating strategy for CD8+ lymphocytes.

<p>These plots represent the gating strategy used for flowcytometry analysis of blood samples; A, lymphocytes were gated on a forward scatter area versus side scatter area dotplot; B, CD8+ bright lymphocytes were gated for further determination of subpopulations; C, CD45RO- and CD45RO+ events were gated to determine naïve and memory subpopulations of CD8+ bright lymphocytes; D, activation of memory CD8+ T cells was determined by determining the percentage of CD38 and HLA-DR double positive CD8+ memory lymphocytes. Maturational T cell subpopulations were identified by analysing the (co-)expression of CCR7 and CD27 within E, naïve and F, memory CD8+ bright lymphocytes.