RT-qPCR validation of three differentially expressed genes within PBMC and WB subgroups, within SOFA groups.

<p>Darker colors correspond to higher magnitude of fold change. Fold changes showed similar patterns between all 10 genes, 9 of which are down-regulated, and 1 of which is up-regulated. 7 of 10 genes showed similar patterns between PBMC and WB and also within SOFA score groups according to our microarray and PCR results. 2 of 10 genes (IL11RA and GRB10) show an opposite result in HIGH PCR (PBMC) and low/med array (WB) correspondingly. Only 1 gene, MAP4K1, shows an opposite result between SOFA score groups.