miR-503 targeted L1CAM in osteosarcoma cells.

<p>(A) The sequences of miR-503 binding sites within the human L1CAM 3'UTRs and schematic reporter constructs, in this panel, L1CAM -WT represent the reporter constructs containing the entire 3'UTR sequences of L1CAM. L1CAM -MUT represent the reporter constructs containing mutated nucleotides. (B) The analysis of the relative luciferase activities of L1CAM -WT, L1CAM -MUT. The error bars are derived from triplicate expriments. (C) qRT-PCR analysis of L1CAM mRNA expression in the Mg-63 cells after treatment withmiRNA mimics or scramble or no transfection. The expression of L1CAM was normalized to GAPDH. (D) Western blot analysis ofL1CAM expression in the MG-63 cells transfected with miR-503mimics or scramble or no transfection. GAPDH was also detected as a loading control.***p<0.001.