NOTCH3 is a target of <i>miR-1</i>.

<p>(A) <i>miR-1</i> is downregulated in human colorectal cancer tissues. qRT-PCR was performed with 27 pairs of human normal and tumor colon tissues. (B) DLD-1 cells were transfected with Pre-miR-control or -miR-1, and subjected to immunoblotting analysis with the indicated antibodies (Left) and qRT-PCR analysis of the indicated genes (Right). α-tubulin was used as a control. (C) The predicted <i>miR-1</i>-binding site in the <i>NOTCH3</i> 3’UTR. Sequences of <i>miR-1</i> and wild-type (WT-3’UTR) and mutated (Mut-3’UTR) <i>miR-1</i>-binding sites are shown. Mutated bases in Mut-3’UTR are marked by gray boxes. Bases in WT- and Mut-3’UTR complementary to those in <i>miR-1</i> are indicated by lines. (D) DLD-1 cells were co-transfected with a reporter plasmid and Pre-miR-control or -miR-1, and lysates were subsequently assayed for luciferase. *<i>p</i> < 0.05.