Cell viability and cFOS mRNA expression in PHN cultures after incubation with C75 or FSG67.

<p>PHN immunostains showed (A) 85% MAP2-positive neurons, 0.41% OX42-positive microglia, and (B) 4.8% GFAP-positive astrocytes. Hoechst 33342 stained nuclear DNA. Treating PHN with (C) 70 µM C75 or (D) 160 µM FSG67 for 24 h did not alter cell viability. Acute treatment of PHN with (E) C75 increased cFOS mRNA, but (F) FSG67 did not alter cFOS mRNA expression. Cell viability data were from two experiments, four to six replicates each. cFOS data were from two independent experiments, each with two or three replicates. For all data: **, <i>p</i><0.01; *, <i>p</i><0.05. Data are represented as means ± SEM.